Abstract: The South-to-North Water Diversion Project is a major national water conservancy project, but the effect of the water transfer depends on the utilization of water in the receiving areas. Water Diversion and Source Supplement Project is a specific utilization of the South-to-North Water Diversion Project in Zouping City. In order to analyze the effect of Water Diversion and Source Supplement Project on groundwater recharge in Daixi River Basin, the authors adopted the water balance method to calculate the groundwater recharge and total recharge of water diversion sources, based on the analysis of the groundwater recharge items in Daixi River Basin after carrying out this project. The total recharge is regarded as the amount of exploitable groundwater resources. The exploitable potential of groundwater resources in this area is evaluated by the comparison of the amount of exploitable groundwater resources and the actual amount of groundwater exploitation, and the effect of Water Diversion and Source Supplement Project on the over-exploitation of groundwater on the remission area was also demonstrated. Besides, the effective replenishment effect of this project on the groundwater in the study area was also revealed based on the annual groundwater and multi-year groundwater level dynamic monitoring data, which provides some reference for the rational development and utilization of groundwater resources in this region.