Abstract: In order to analyze the distribution and fluids hydrochemistry characteristics of hydrothermal geothermal resources in Guizhou Province, the authors investigated and sampled fluids from hot springs and geothermal wells within the province. Based on the previous and newest geothermal resources exploration data,through geothermal resources exploration and evaluation standard and Piper Graph of fluids hydrochemistry, the authors divided geothermal resource types, and analyzed resource distribution, resource enrichment and geothermal reservoir characters. The characteristics of fluids hydrochemistry and trace elements in different tectonic zones and different geothermal reservoirs were also studied in this paper. The results show that the hydrothermal geothermal resources in Guizhou Province are mainly distributed in Central and Northern Guizhou, which belong to low temperature geothermal resources. And the main resource type is uplift (fold) fracture type and the most widely distributed geothermal reservoirs are layered reservoir and banding reservoir. The enrichment and outcrop of geothermal fluids are obviously controlled by the structure. The biggest four geothermal resource areas, mainly Sinian and Cambrian dolomite geothermal reservoirs in a stable dyuamic state, are Shiqian, Zunyi, Guiyang and Xifeng. The hydrochemistry characteristics of the fluids in same tectonic zones and geothermal reservoirs are basically same. The fluids belong to bicarbonate type in hudrochemistry and they have low mineralization, and high content of trace elements and radioactive elements, such as strontium, metasilicate, fluorine and radon.